The Department's of Biochemistry and Biotechnology of St. Joseph's College for Women,  Visakhapatnam,  in collaboration with TCABS-E are organizing a 2 day virtual National workshop on " Insights into Bioinformatics Tools and Databases to Analyze Biological Data" during the date - 20th and 21st November,  2021.

ABOUT THE COLLEGE

St. joseph’s college for women was Started by   Sisters  of  St. Joseph of Annecy  in  India,  is  the  year  1958,  exclusively  for  women,  the first of  its kind in Visakhapatnam.

In the 50s, Visakhapatnam did not have any facility for higher education of young women. So impressed were the public of Visakhapatnam with the service rendered by the Sisters that, in 1957, a group of prominent citizens requested the Sisters of St. Joseph to undertake the task of setting up an institution of higher learning for the young women of the city.

The college, affiliated to Andhra University, was founded on 2nd July 1958.

The college is now a grant-in-aid institution, with a campus area of 7.5 acres and student strength of 2500, offering Three Intermediate courses, 12 Undergraduate courses in BA, B.Com, B.B.A and B.Sc and Six Postgraduate courses.  The UGC, recognising the outstanding quality of education and excellent academic record,  conferred Autonomy on the College in 1987.

The college is a member of the All India Association of Christian Higher Education as also the Xavier Board and utilizes the facilities of these agencies for training the faculty. The Department of French has linkages with the educational wing of the French Embassy, New Delhi and Alliance Francaise, Hyderabad. The Department of Home Science has a Memorandum of Understanding with the Community Foods and Nutrition Unit, Ministry of Human Resource Development, for training the Home Science students in the techniques of Fruit and Vegetable Preservation.

Program sheet for 20-11-2021

6.0 pm – Invitation to the program by Dr P. Mary Anupama

6.02pm – Prayer song by Ms Bhoomija

6.04pm – Introduction to college and departments by Dr Anupama

6.08 pm- Introduction to workshop by Dr T. Verabrahmam , In charge head of Biotechnology

6.10 pm by the Founder of TCABS-E- Dr Ravi Kiran Yedidi

REPORT

20^th November, 2021. Time – 6 to 7.30

Ms Madhumita Aggunna, Hod of Mutiomics-Oncology & Immunotherapy

Topic- Introduction to Bioinformatics NCBI Navigation & Databases

The talk started with introduction to The National Center for Biotechnology Information advances science and health that provides access to biomedical and genomic information.

https://www.ncbi.nlm.nih.gov/.

It has primary and secondary data bases and scientists dump first in primary database and secondary- data which is analyzed after deposition

We can select the sublinks for DNA or protein sources while many toher formats also exist. Next is PUBMED- easy to find recent research using filters. Filter help us to sort out and make search easy while PUBMED central gives free articles.

Ms Niharikha Mukala, HoD of Multiomics- Infectious Disease

Topic- Data Analysis

In search type the gene, It displays gene id, description, location, locus tag

Annoted genes are coded genes and Ref sequence- annoted and accessible genes

XC/XR/XP are synthetic genes.

Upon retrieval the following are seen-

Green bar- complete gene sequence

Red bar- protein binding site

Golden brown- recombinant site-

Black- annoted protein

She talked about Gen bank and how to retrive and track the latest publication too.

The difference between pub med and pubmed central was explained

Changing BLAST sequence and all the related aspects were very well narrated

Day 21-11-2021

Ms Madhuri Vissapragada, HoD of Synthetic Biology

Topic- BLAST

Blast- Basic local alignment tool. It is used to find regions of local similarity

Sequences of nucleotides and proteins are available. Give- query which is compared to set of sequences in databases- it gives statistical details of comparison

Features of blast were dealt- Different kinds of blast features are there- Ig BLAST is latest. Nucleotide and protein blast are frequently used

Nucleotide blast- - give n. Nucleotide sequence has 6 open reading frames- Blast X takes all the six possible frames and compares with proteins

tBlastn- all possible reading frames are compared to all available nucleotide sequences.

There are specialized search features in BLAST

Run blast by giving entire sequence- choose option, give job title and run blast.

E value= M x n x 2power bit score

E value less than 1 is good hit

% similarity - % identity

Download description table so that all description of all will be displayed with hyper links

Graphic summary- Alignments helps us to understand similarity and also evolution relation

100% alignment- red bar

Less alignments changes color of bar

Alignments- give sequences with nucleotide to nucleotide alignment

Ms. Santhi Nissi Addala, HoD Stem Cell Biology

Topic- Global Align & Clustal Omega

Sequence alignment- it is used to identify sequence similarity, evolution relationships etc

2 types- pair wise and multiple sequence alignment

Pair wise- one pair at a time- 2 type- global and local alignment

Multiple allows multiple sequence at single stretch

Global alignment-  was displayed and narrated with all results and options